【摘要】 对固定化酵母生物合成胞二磷酸胆碱反应液浓度进行了研究 ,通过几组实验 ,找到了反应液中底物的最佳浓度 :磷酸胆碱 75 μmol/mL ,胞苷酸 (CMP) 10 μmol/mL ,葡萄糖 4 0 0 μmol/mL ,硫酸镁和硫酸锰分别为 10 μmol/mL和 1μmol/mL ,可以不使用还原剂。更多还原

【Abstract】 The reaction solution concentration for biosynthesis of cytidine-5’-diphosphate choline by immobilized yeasts was studied. Throught experiments,the optimum concentrations of substrates in reaction solution were as follows:phosphocholine,75μmol/mL;CMP,10μmol/mL;glucose ,400μmol/mL;MgSO 4,10μmol/mL; MnSO 4,1μmol/mL and no reducer.更多还原