【摘要】 建立了一种表面活性剂TritonX -1 0 0存在下 ,用 2 -(5 -溴 -2 -吡啶偶氮 ) -5 -二甲氨基酚 (简称 5 -Br-DMPAP)作显色剂直接光度法测定精液锌含量的新方法。该法显色络合物最大吸收波长为 5 5 6nm ,线性范围达 4 5 9mmol·L- 1,表观摩尔吸光系数为 1 1 2× 1 0 5L·mol- 1·cm- 1,回收率为 97 3 %～ 1 0 4 2 % ,批内和批间变异系数 (CV)分别为 3 1 %与 4 2 % ,与原子吸收分光光度法比较相关良好 ,y =0 984x +0 0 1 ,x =0 9869,P >0 0 5 (n =3 2 )。该法具有操作快速、简便、结果灵敏可靠等优点 ,适合在临床上推广应用更多还原

【Abstract】 Zinc in seminal fluid was determined by UV-spectrophotometry with 5-Br-DMPAP.The maximum absorption of the compound was at 556 nm.The linearity of test was up to 4 59 mmol·L -1 The molar absorption coefficient is 1 12×10 5 L·mol -1 ·cm -1 .The recoveries ranged from 97 3% to 104 2%.The within-run and between-run CVs are 3 1% and 4 2%.Comparing this method ( y )with AAS ( x ) the regression equation is y =0 984 x +0 01, r =0 9869, P >0 05 This method is simple,sensitive,rapid and acurate.The method is suitable for clinical application.更多还原