【摘要】 本文对在酸性溶液中牛血清白蛋白与酸性铬兰K结合反应进行了研究。在pH 2 87盐酸 乙酸钠缓冲体系中 ,随着BSA浓度的逐渐增大 ,酸性铬兰K在 5 2 3nm的吸光度下降 ,吸收峰向长波方向移动。研究表明牛血清白蛋白与酸性铬兰K在通过静电引力而结合 ,结合反应符合相分配模型 ,并探讨了酸度、试剂浓度、离子强度对结合反应以及表观结合常数Kc、结合数n ,Sandell灵敏指数的影响 ,对反应时间、表面活性剂对体系的影响以及工作曲线进行了研究。更多还原

【Abstract】 This paper investigated the interaction of acidic chrome blue K with bovine serum albumin (BSA). When BSA was added into acidic chrome blue K solution at pH 2 87 HCl NaAc buffter, bathochromic effect and hypochromicity were observed. With the increase in BSA concentration, the absorption peak at 523 nm decreased. It was considered that the combination of BSA with acidic chrome blue K is due to static electricity forces. The interaction is in accord with model of phase distribution. It was discussed the effect of acidity, concentration of acidic chrome blue K, ion strength to apparent binding constant K c, binding number n , Sandell constant. The reaction time, surfactant, work curve were studied.更多还原