【摘要】 研究了异硫氰酸荧光素 (FITC)标记的羊抗人抗体 (GAHAb FITC)和兔抗羊抗体 (RAGAb FITC) ,及其以三种免疫方式与人免疫球蛋白 (IgG)反应所得免疫复合物在多种固体薄膜基质上发射室温磷光 (RTP)的适宜条件及其光谱、强度和寿命等性质。研究发现 ,标记抗体及其免疫复合物保留了FITC优良的固体基质室温磷光性质 ,λ(max)ex λ(max)em =5 2 5 6 5 0nm ,其RTP强度与其浓度线性相关 ,并有很高的灵敏度。更为重要的是 ,免疫反应及其RTP检测能结合于同一基质 ,方便地相继完成。在聚酰胺膜 (PM )上 ,以Pb(Ac) 2 为重原子微扰剂 ,稀释比为 1∶10 (φ)的GAHAb FITC ,RAGAb FITC及其与人IgG形成的免疫复合物均能发射较强的RTP信号并有较长的RTP寿命。本结果为建立相应的固体基质室温磷光免疫分析 (SS RTP IA)新方法奠定了相应的实验基础。更多还原

【Abstract】 The properties of room temperature phosphorescence (RTP) of the labeled goat anti human antibody(with fluorescein isothiocynate GAHAb FITC)and labeled rabit anti goat antibody(RAGAb FITC) with FITC in different dilution ratios on many kinds of solid substrates were studied in detail. It is found that on the polyamide membrane, only using Pb(Ac) 2 as a heavy atom perturber, both the labeled antibodies and their antigen antibody conjugated compound with the human immunoglobulin G remained excellent RTP properties of isothiocyanate fluorescein, λ (max) ex / λ (max) em =525/650 nm, the relation of RTP intensity to the concentration was linear in a certain range.The conditions for immunological reaction and RTP emission were optimized. The result means that it is possible to develop a new immunoassay named as solid substrate room temperature phosphorescence immunoassay (SS RTP IA), which combines both the specificity of immunological reaction and high sensitivity of luminescence analysis.更多还原