【摘要】 综述了国内外果树种质离体保存方面的研究进展,简单介绍了种子保存,常规的细胞、组织和器官保存方法。超低温保存是一项长期稳定保存种质的方法,能减慢细胞代谢和完全抑制生长,概述了超低温保存的原理、技术要点和主要优点,以及影响超低温保存的因素:包括植物材料的性质、预处理、冰冻保护剂种类、降温方法、解冻方法以及解冻后处理等,并对今后果树种质保存提出了一些建议。更多还原

【Abstract】 The latest achievements of the research in in vitro conservation of fruit germplasm are summarized in this paper. Fruit seeds preservation and some common methods of fruit tree cell, tissue and organ preservation are briefly mentioned. The theory,affecting factors,key technology and the main merits of cryopreservation,an ideal method for long-term conservation for fruit germplasm are introduced in details. Some factors which affect the results of cryopreservatoin, including material characters, pretreatment, cryprotectants, freezing and thawing methods are discussed as well. Some points for improving fruit germplasm conservation in the future are recommended.更多还原