【摘要】 紫膜中的蛋白质细菌视紫红质 (bR)具有独特的光循环和光致变色特性 ,在分子电子学和生物电子技术领域具有广泛的潜在应用价值 .本文采用稀土纳米晶CeO2 对细菌视紫红质 聚乙烯醇 (bR PVA)薄膜进行化学修饰 .研究了纳米晶对bR光循环中的重要中间态M态的寿命的影响 .发现上述稀土纳米晶可延长M态寿命 ,且晶粒尺寸越小对M态寿命的影响越大 .纳米晶周围的羟基有助于阻碍席夫碱获得质子 ,从而使M态寿命延长更多还原

【Abstract】 Bacteriorhodopsin,which consisted in the purple membrane,has unique photocycle and photochoromism properties and can be widely used in moleculer electronics and bioelectrotechnics.Bacteriorhodopsin polyvinyl alcohol (bR PVA)films were prepared with CeO 2 nanocrystallines as chemical additives.The effect of the nanocrystallines on the lifetime of M state,which is an important intermediate in the photocycle of bR,is investigated.It is observed that the lifetime of M state can be prolonged by rare earth nanocrystalline,and the lifetime of M state increases with the decreasing size of CeO 2 nanocrystalline.Hydroxyl groups around the nanocrystallines help to prevent the Schiff base in the M state from uptaking protons,and the decay rate is retarded and the lifetime of the M intermediate is prolonged.更多还原