【摘要】 目的 探讨过氧化氢（hydrogen peroxide,H₂O₂）对体外培养的人胚胎视网膜色素上皮（RPE）细胞的抑制作用及DNA合成的影响。方法 分别用MTT法测定加入0、50、100、200、400、500μmol/L的H₂O₂作用60h后RPE细胞数量的改变和核酸蛋白分析仪测定加入50、100、200、300、400、500μmol/L H₂O₂作用60h后RPE细胞NDA浓度的变化。结果50、100、200、400、500μmol/L的H₂O₂作用60h后其细胞A值分别为（0.1731±0.0260）、（0.1590±0.0418）、（0.1491±0.0265）、（0.1506±0.0224）、（0.1538±0.0221）与对照组（0.1978±0.0223）相比,差异有显著性（/值分别为1.961、3.082、3.868、3.749、4.932,P值分别为0.054、0.003、0.000、0.000、0.000）;50、100、200、300、400、500μmol/L H₂O₂作用60h后其细胞DNA浓度分别为（247.5225±36.8007）、（138.3373±14.6158）、（1043.3095±14.2524）、（85.6586±14.8986）、（70.2618±11.8879）、（56.1955±12.8999）μmol/L,与对照组（293.9311±51.7510）μmol/L相比,差异有显著性（t值分别为3.659、12.376、15.083、16.566、17.791、18.909,P值分别为0.001、0.000、0.000、0.000、0.000、0.000）。结论H₂O₂能抑制体外培养的RPE细胞增殖及DNA合成。更多还原

【Abstract】 Objective To investigate the inhibitive effects of hydrogen peroxide (H2O2) on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium (hRPE) cells in vitro. Methods Cultured hRPE cells were treated with H2O2 by various concentrations for 60h; changes in the number of RPE cells and in DNA contents in RPE cells were determined respectively using the methyl thiazolyl tetrazolium (MTT) assay method and Nucleic Acid & Protein Analysis method. Results The A values of hRPE cells treated with 50, 100, 200, 400, 500 μmol/L H2O2 were respectively (0.1731±0.0260), (0.1590±0418), (0.1491±0.0265), (0.1506±0.0224) and (0.1538±0.0221) fimol/L and they were significantly different from that in the control (0.1978±0.0223), ( t being 1.96, 3.082, 3.868, 3.749 and 4.932 respectively, P being 0.054, 0.003, 0.000, 0.000 and 0.000 respectively). The DNA contents in hRPE cells treated with 50, 100, 200, 300, 400 and 500 μ mol/L H2O2 were respectively (247.5225±36.8007), (138.3373±14.6158), (104.3095±14.2524), (85.6586±14.8986), (70.2618±11.8879) and (56.1955±12.8999) μ mol/L and were significantly different from that in the control (293.9311±51.7510) (t being 3.659, 12.376, 15.083, 16.566, 17.791 and 18.909 respectively. P being 0.001, 0.000, 0.000, 0.000, 0.000 and 0.000). Conclusion H2O2 can inhibit the proliferation and DNA synthesis in cultured hRPE cells in vitro in a dose-dependent manner.更多还原