【摘要】 以皱纹盘鲍 (HaliotisdiscushannaiIno)成鲍为对象 ,采用“平珠”技术 ,将圆形玻片插入鲍鱼的外套膜外腔 ,配制了 2种不同磷含量的半精制饲料 ,在缺磷的人工海水中喂养鲍鱼 4 0天。养殖结束后取出“平珠” ,利用扫描电镜对其超微结构进行了观察 ,并与正常贝壳作比较。结果发现 :磷添加饲料组所得“平珠”具有与正常贝壳一致的矿化结构 ,而缺磷饲料组所得“平珠”晶体的形成、生长和结构都受到显著影响 ,方解石的排列失去规律 ,文石的晶态也变得无规则。本研究证明了“平珠”生成法能够模拟贝壳的自然生长 ,是研究贝壳生物矿化及其营养学机理的有效途径。同时证明了磷对于维持贝壳的晶体形成、正常生长和结构是必不可少的。更多还原

【Abstract】 A flat pearl culture method was used to investigate the effects of dietary phosphorus on the shell biomineralization in abalone Haliotis discus hannai Ino. Glass coverslips were inserted between the mantle and the inner surface of the shell of abalone. Animals were fed semi purified diets with 2 levels of supplemented phosphorus,and reared in phosphorus free artificial seawater for 40 days. Ultrastructures of shells and flat pearls were analyzed with scanning electron microscopy (SEM). Results showed that minearlizated structures of flat pearls in phosphorus supplemented treatment were in line with those of normal shells. However, arrangement and morphology of calcite and aragonite on flat pearls in phosphorus deficiency treatment were irregular. It is demonstrated here that the flat pearl culture can be used to study the nutritional mechanism of shell biomineralization. Dietary phosphorus is essential to shell biomineralization.更多还原