【摘要】 采用 pET2 8a载体系统在E .coliBL2 1中进行了植物毒素gelonin的原核表达 ,产物经SP Sepharose ,Superdex 75二步纯化 ,获得了电泳纯的重组植物毒素 gelonin。将重组的gelonin与从植物种子中提取的天然 gelonin进行了Westernblot,ELISA ,无细胞体系中蛋白质合成抑制实验以及超螺旋DNA裂解研究。结果都表明 ,重组植物毒素gelonin与天然 gelonin具有相似的生物活性更多还原

【Abstract】 The plant toxin gelonin was expressed in E.coli using a plasmid pET-gel which contains the coding region of gelonin in vector pET-28a. Recombinant gelonin was purified by two steps, i.e. SP-Sepharose and Superdex 75 chromatography. Functional studies pointed out that this protein behaves similarly to native product as determined by Western blot, ELISA, cell free protein synthesis system and cleavage of supercoiled DNA.更多还原