【摘要】 研究和比较了止咳枇杷冲剂中桑白皮、白前和百部中特征化学成分的提取和薄层色谱鉴别的条件。分别取桑白皮、白前、百部药材和止咳枇杷冲剂及各被鉴别药材阴性制剂的提取液 ,桑白皮在聚酰胺板上 ,以醋酸展开 ,365nm紫外光下观察 ,得两个蓝绿色特征荧光斑点 ;白前和百部在硅胶板上 ,前者以 ψ(氯仿 ∶甲醇∶水 ) =2 ∶1∶1 .5展开 ,磷钼酸显色 ,得两个红色斑点 ,百部以 ψ(正己烷 ∶氯仿∶甲醇 ) =5∶5∶0 .2展开 ,硫酸乙醇显色 ,得两个棕红色斑点 ;在阴性制剂色谱图中两者均无此类斑点。因此该方法可作为该制剂中这三味中药的鉴别方法研究和比较了止咳枇杷冲剂中桑白皮、白前和百部中特征化学成分的提取和薄层色谱鉴别的条件。分别取桑白皮、白前、百部药材和止咳枇杷冲剂及各被鉴别药材阴性制剂的提取液 ,桑白皮在聚酰胺板上 ,以醋酸展开 ,365nm紫外光下观察 ,得两个蓝绿色特征荧光斑点 ;白前和百部在硅胶板上 ,前者以 ψ(氯仿 ∶甲醇∶水 ) =2 ∶1∶1 .5展开 ,磷钼酸显色 ,得两个红色斑点 ,百部以 ψ(正己烷 ∶氯仿∶甲醇 ) =5∶5∶0 .2展开 ,硫酸乙醇显色 ,得两个棕红色斑点 ;在阴性制剂色谱图中两者均无此类斑点。因此该方法可作为该制剂中这三味中药的鉴别方法。更多还原

【Abstract】 Objective:To study a specific TLC identification of the Mulberry Bark(MB) and the Swallowwort Rhizome(SRh) and the Stemona Root(SR) in the cough infusion of loquat leaf. Methods:The extracts of MB & SRh & SR and their preparation were subjected to TLC in different development systems. The TLC plat of MB was examined under short wavelength UV light. The TLC plat of SRh. colored two same orange red spots with its preparation by Phosphomolybdic acid ethanol solution. The TLC plat of SR colored two same brownish red spots with its preparation by sulphuric acid ethanol solution. Results:A solution of acetic acid was used for development and the plant of MB was examined under UV light of 365 nm. Specific fluorometric spots of the certified MB were found. A mixture of chloroform and methanol and water(2 ∶1∶ 1.5) was used for development and phosphomolybdic acid ethanol solution is used to color, specific orange red spots of the certified SRh were found. And a mixture of chloroform and methanol and n hexane(5 ∶0.2∶ 5) was used for development and sulphuric acid ethanol solution to color, specific brownish red spots of the certified SR were found. Conclusion:The specific spots showed by TLC can be regarded as the identification of SRh & MB & SR in the cough infusion of loquat leaf.更多还原