【摘要】 合成了适于反胶束法提取蛋白质的磷酸酯表面活性剂二油酸乙二醇单酯基磷酸酯钠 (DEOPA) ;以氢核磁共振谱和红外光谱表征了其结构 ;测定了其临界胶束浓度 (cmc)、亲水亲油平衡值 (HLB值 )及对水的增溶作用等表面化学性质 ;考察了 DEOPA/异辛烷反胶束体系提取蛋白质的性能 .结果表明 ,DEOPA具有较高的表面活性 ,适合于构建反胶束体系 ,并可用于提取大分子量的蛋白质更多还原

【Abstract】 A new phosphate surfactant that has never been reported before, sodium di-ethyloleyl phosphate acid(DEOPA), which fits for the extraction of proteins with reversed micellar system, has been synthesized. Its structure has been characterized with 1H NMR and FTIR spectrum. Its surfacial physico-chemical properties, such as cmc(Critical Micellar Concentration), HLB(Hydrophilic-Lipophilic Balance), and its solubilization effect to water was detected. Extraction of proteins using reverse micelles composed of DEOPA and isooctane was carried out. The results show that DEOPA is of relatively high surfactant activity, which means it fits for composing reversed micellar systems, and is of superiority when extracting large molecular weight proteins.更多还原