【摘要】 重症肌无力患者体内致病毒素是乙酰胆碱的受体抗体 .通过对载体材料的优化及对配基的筛选发现 ,使用质量分数为 6%的纤维素球形载体 ,以环氧氯丙烷法活化并固定上 L-色氨酸作为配基制备的血液灌流材料在静态吸附实验 (1 g吸附剂加入 3 m L 病人血清 ,室温 ,3 h)中可使抗体水平下降 2 5 %以上 ,吸附量达3 .669× 1 0 - 3nmol/g,选择性较高 ,具有应用前景更多还原

【Abstract】 Myasthenia Gravis(MG) is an serious nerval specific autoimmune disease. The pathogenic factor of the disease is the anti-AChR antibodies in the blood of patients which react against the acetylcholine receptor(AChR). Literature reported that the AChR ab could be adsorbed by adsorbent linked with special amino acids or proteins. In order to remove AChr ab from blood, we studied the selectivity and adsorption capacity of various ligands and matrixes. Experimental results showed that immunoadsorbent prepared by coupling L-tryptophan to epichlorohydrin-activated cellulose bead(6%) gave the best result. Adsorbent of 1.0 g in 3.0 mL of serum could remove 25% of antibody from the serum, which will have a high potential application in the future.更多还原