【摘要】 用荧光光谱法研究了毒蛋白 Cry Ab在溶液中的构象 .结果表明 ,p H值增大时 ,毒蛋白的荧光强度也增大 ,至 p H=1 1时荧光强度已达最大值 ;甲醇、乙醇及异丙醇等有机溶剂引起毒蛋白荧光发射波长蓝移和荧光强度增大 ,至有机溶剂的浓度超过某一阈值后荧光发射波长不再蓝移 ;丙酮能完全猝灭毒蛋白Cry Ab的荧光 ,丙烯酰胺能猝灭 Cry Ab86%的荧光 ,而 KI对毒蛋白的荧光无猝灭作用 ,由此推断 Cry Ab的大部分色氨酸残基位于分子表面富含负电荷的区域中更多还原

【Abstract】 The conformations of the toxic protein CryⅠAb in its solution were studied by fluorescence. The results showed that the toxic protein fluorescence intensity is improved with its pH value increase and reaches its maximum at pH equivalent to 11. Adding some organic solvents such as methanol, ethanol, isopropanol result in the emission wavelength of fluorescence of the toxic protein blue shift and its fluorescence intensity increase. However, the toxic protein fluorescence emission wavelength no long blue shifts as these organic solvents concentration beyond certain values. Acetone can quench all fluorescence of the toxic protein CryⅠAb and acrylamide can quench 86% of the total fluorescence of the toxic protein, but KI can′t quench any fluorescence. It implicates that the most of tryptophan residuals of CryⅠAb are located on the toxic protein surface where the negative charge is rich.更多还原