【摘要】 <正>加替沙星(Gatifloxacin,GFLX),卜环丙基－6－氟－7－(3－甲基－1赈嗓基)－8－甲氧基－1,个二氢－个氧－隆琳－3－竣酸1/2水合物是一种新型的第三代氟哆诺酮抗菌药物,与现有的氟喳诺酮类药物相比,更多还原

【Abstract】 A selective and sensitbe sensitized chemiluminescence （CL） method for the determination ofGatifloxacin（GFLX） is described. The method is based on Tb3+ sensitized chemiluminescence of the Tb3+-GFLX-Ce（Ⅳ）-SO3₃^2- system since CL for the binary Ce（Ⅳ）-SO₃^2- system is weak, while CL is enhancedwhen Tb3+-GFLX is added to the Ce（Ⅳ）-SO₃^2- system. The possible mechanism of the CL reaction maybe that Ce（Ⅳ） can oxidize HSO₃^- to a hydrogen-sulfite radical HSO3, then HSO3 radicals produce S2O₆^2-,S2O₆^2- will form the excited intermediate product SO2. The energy of SO2 is mainly transferred to Tb3+-GFLX, and the CL spectrum shows a characteristic emission of Tb3+. The re1ationship between the CL in-tensity and the concentration of GFLX is linear in the range of 2.0 × 10-8-1.0×10-6 mol/L, and the de-tection limit is 3. 5 × 10-9 mol/L. The relative standard deviation（RSD） for 11 independent determinationsof 1.0 X 10-7 mol/L GFLX was 3.2%. Common excipient starch was used in pharmaceutical preparationand most of metal ions do not interfere with the determination of GFLX. GFLX in urine can be analyzeddirectly after appropriate dilution of the samples, and the analytical result is satisfactory.更多还原