【摘要】 用激光诱导荧光检测的高效毛细管电泳结合微透析技术测定急性力竭运动前后的大鼠下丘脑区细胞外液中的氨基酸神经递质的含量 .实验结果表明 ,急性力竭运动后大鼠下丘脑区的细胞外液中的 6种氨基酸 (精氨酸、甘氨酸、赖氨酸、谷氨酸、丙氨酸、γ-氨基丁酸 )有统计意义的增加 ,这一结果显示了下丘脑区的氨基酸在应激过程中很可能起着重要的作用更多还原

【Abstract】 A simple and effective coupling of microdialysis and capillary electrophoresis with laser induced fluorescence detection technique was applied to the analysis of amino acid neurotransmitters in hypothalamus of rat after an acute exhausting exercise. The separation of amino acids was achieved by using an uncoated fused silica capillary(57 cm×75 μm i.d.) with a buffer of 10 mmol/L disodium tetraborate at pH 10 and an applied voltage of 12.5 kV. The detection limit was 10 -10 mol/L for each amino acid. It is sufficiently sensitive and rapid for the determination of amino acid in 5 μL Microdialysate. In comparison to pre exercise, a significant increase in the levels of 6 hypothalamic amino acids(arginine, glycine, lysine, glutamic acid, alanine, γ amino n butyric acid) after exercise was found. These results demonstrate that increase of metabolic amino acids in hypothalamus of rats can be induced by exhaustive exercise and suggesting that amino acid neurotransmitters may play functional roles in the central effects of exercise.更多还原