【摘要】 以聚甲基丙烯酸环氧丙酯微球 ( PGMA)为基质 ,利用还原氨化方法将肝素键合在其表面上 ,得到一种以肝素为配基的高效亲和色谱填料 ,该填料对抗凝血酶 的亲和容量为 1 .2 mg/g,对抗凝血酶 和人血清白蛋白的回收率分别为 96.9%和 94 .4 % .利用前沿色谱法测定了肝素与抗凝血酶 之间的表观解离常数为 1 .96× 1 0 - 5mol/L.利用该填料可直接从人血浆中分离出抗凝血酶更多还原

【Abstract】 A high performance affinity chromatography medium used for separation of antithrombin Ⅲ was prepared. Monodisperse poly(glycidyl methacrylate) beads(PGMA) were employed as the affinity support. Heparin was linked covalently to amino PGMA beads, which was prepared by amination of PGMA. The resultant affinity media present a minimal non specific interaction with proteins and the protein recovery was 96.9% and 94.4% for antithrombin Ⅲ and human serum albumin, respectively. Its affinity capacity for antithrombin Ⅲ was 1 2 mg/g beads. The apparent dissociation constant between antithrombin Ⅲ and immobilized heparin was 1 96×10 -5 mol/L which was evaluated by front chromatography. The affinity medium was used to separate antithrombin Ⅲ from human plasma.更多还原