【摘要】 提出利用对肼基苯磺酸作为糖的衍生试剂 ,以实现快速的毛细管电泳分离与检测 .详细研究了影响衍生反应的诸多因素以及衍生产物的毛细管电泳分离条件 .结果表明 ,本方法可以在温和条件下实现快速反应 ( 1 0 min) ,2 0 0 nm紫外检测下葡萄糖的最低检出量可达 1 7.6fmol,检出浓度 3.6μmol/L.在 1 0 0mmol/L H3BO3( p H=1 0 .2 4 )体系中实现了 9种单糖和二糖的高效分离更多还原

【Abstract】 p Hydrazinebenzenesulfonic acid was explored as a novel pre capillary labeling reagent for CE of mono and disaccharides. The derivatization and the separation in borate buffer were investigated in detail. The derivatization took less than 10 min and the mass detection limit at 200 nm reaches 17.6 fmol of glucose, corresponding to concentration limit of 3.6 μmol/L. The baseline separation of 9 mono and disaccharides could be achieved within 11 min, using a running buffer composed of 100 mmol/L borate at pH 10.24. This approach is also suitable for quantitation with linear coefficients higher than 0.992 at the concentrations between 0.1-9.1 mmol/L.更多还原