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人胎肺Ⅱ型肺泡细胞中表面蛋白-B的表达及意义

Expression of pulmonary surfactant protein-B and its regulative function in alveolar type Ⅱ cells of human fetal lungs

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【作者】 熊轶黄中新覃莉

【Author】 Xiong Yi, Huang Zhongxin, Qin Li.Department of Histology & Embryology, Medical College, Jinan University, Guangzhou 510632 China

【机构】 暨南大学医学院组织胚胎学教研室暨南大学医学院组织胚胎学教研室 广州 510632广州 510632广州 510632

【摘要】 目的检测肺表面蛋白-B(SP-B)及其调控因子甲状腺转录因子-1(TTF-1)在肺泡Ⅱ型细胞发育过程的表达特征,探讨两者对于人胎肺肺泡Ⅱ型细胞发育、分化和成熟的调控作用。方法取16-35周人胎肺组织,常规石蜡包埋切片,用免疫组化技术检测SP-B和TTF-1的表达特征。结果,TTF-1在胎肺16周开始表达,定位于上皮细胞核内,随支气管树的发育分化,远端位置的反应总是较近端者强。SP-B蛋白于18周开始表达,定位于肺泡Ⅱ型细胞胞浆内,阳性反应细胞于早、中期表达丰富;由呼吸道近端逐渐往远端迁移,且强度不断增强。发育末期至成肺中,TTF-1和SP-B阳性反应均在肺泡Ⅱ型细胞中稳定表达。结论TTF-1参与支气管树形态发生和肺泡的发育成熟,并调控肺泡Ⅱ型细胞中SP-B蛋白的分泌,起稳定肺泡直径的作用。因此,SP-B蛋白的分泌反映肺泡Ⅱ型细胞功能的成熟。

【Abstract】 Objective To observe the distribution of surfactant protein-B and its regulatory factor-thyroid transcription factor-1(TTF-1) in human fetal lungs; and to study its importance function in regulating lung morphogenesis, differentiation and maturation. Method The 16th - 35th week of human fetal lungs were obtained.The expression of SP-B and TTF-1 were examined by immuno-histochemistry. Result It was shown, that TTF-1 was expressed throughout fetal lung development from 16th week. TTF-1 was prominent in the nuclei of distal lung buds, which was more intense than any of airway epithelium. But SP-B was expressed in cytoplasm from 18th week.The positive reaction of SP-B in distal airway epithelium was more intense than that in proximal ones. From 25th week to newborn and the adult, the expression of TTF-1 and SP-B were both in the cytoplasm of type II cells. Conclusion TTF-1 could stimulate the growth process both of bronchial tree and alveolar type II cells,and regulated the type II cells to secrete SP-B.The expression of SP-B reflected the maturation of the type II cells, and it may be very important for neonate.

【基金】 国家自然科学基金(39770300)
  • 【文献出处】 解剖学研究 ,Anatomy Research , 编辑部邮箱 ,2002年04期
  • 【分类号】R321
  • 【被引频次】10
  • 【下载频次】141
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