【摘要】 利用核磁共振氢谱 (1HNMR)峰面积积分法建立了一种测定庆大霉素C组分比率的新方法 ,可以通过计算准确、快速地得到C1、C1a和C2 各组分的比率。C1、C1a和C2 的相对标准偏差分别为 0 .5 8% ,0 .5 1%和 0 .5 0 %。与常用的高效液相色谱法相比 ,此法具有简单、方便、无需标准品等优点 ,为庆大霉素的质量控制提供了一种新的有效方法。更多还原

【Abstract】 The proportions of gentamicin C components were determined by 1H NMR peak area. The proportions of C 1, C 1a and C 2 can be obtained accurately and rapidly through calculation. The RSDs of C 1, C 1a and C 2 are 0.58%, 0.51% and 0.50%, respectively. Compared with commonly used HPLC, this method is simple and convenient, and does not need standard samples. It provides a new effective way for quality control of gentamicin.更多还原