【摘要】 研究了蛋白质与 7-苯基偶氮 -1 ,8-二羟萘 -3 ,6-二磺酸的显色反应。在 p H2 .7的缓冲溶液中 ,7-苯基偶氮 -1 ,8-二羟萘 -3 ,6-二磺酸与蛋白质形成红紫色复合物 ,λmax为 564 nm,ε为 1 .3× 1 0 5L· mol- 1· cm- 1,线性范围为 6.6～ 1 3 2 mg/ L。实验证实7-苯基偶氮 -1 ,8-二羟萘 -3 ,6-二磺酸与蛋白质的作用符合 Pesavento模式。所提出的方法不经任何预处理可直接测定血清和花生中的蛋白质更多还原

【Abstract】 In a pH 2.7 buffer solution, protein forms a red complex with 7-phenylazo-1,8-dihydroxylnaphthalene-3,6-disulfic acid(PANDS). Its maximum absorption peak is at 564 nm with molar absorptivity of 1.3×10 5 L·mol -1·cm -1. Beer’s law is obeyed in a range from 6.6 mg/L to 132 mg/L. The proposed method has been applied to the determination of protein in serum and peanut samples. For interaction of PANDS and proteins, the Pesavento model is obeyed.更多还原