【摘要】 以邻菲咯啉 (phen)、邻菲咯啉 5 ,6 二酮 (dione)为配体首次合成了高氯酸邻菲咯啉 邻菲咯啉 5 ,6 二酮合铜 。用荧光光谱 ,摩尔比、粘度、MLCT减色效应、平衡常数以及荧光能量转移研究了络合物与鱼精子DNA的结合情况 ,证实了该络合物与DNA存在插入作用。基于络合物对DNA能量转移造成荧光量子产率比值 (Φλ Φ32 0 )的降低 ,解释了不同波长激发光下 ,荧光发射峰在加入DNA后产生猝灭和增强两种绝然不同的现象。更多还原

【Abstract】 A new complex [Cu[(phen)(dione)](ClO 4) 2] was synthesized and characterized (where phen and dione represente 1,10 phenanthroline and 1,10 phenanthroline 5,6 dione respectively). The binding of the complex to fish sperm DNA was investigated by using fluorescence and absorption spectrophotometry, continuous variation method, viscosity measurements, equilibrium binding and the salt dependence of the binding constant and fluorescence energy transfer experiments. The intercalation mode was proved to exist. Especially we found energy could transfer from bound ligand to DNA bases, which provided the additional evidence for the intercalation of copper complex into DNA and was used to explain the fluorescence spectra. was synthesized and characterized (where phen and dione represente 1,10 phenanthroline and 1,10 phenanthroline 5,6 dione respectively). The binding of the complex to fish sperm DNA was investigated by using fluorescence and absorption spectrophotometry, continuous variation method, viscosity measurements, equilibrium binding and the salt dependence of the binding constant and fluorescence energy transfer experiments. The intercalation mode was proved to exist. Especially we found energy could transfer from bound ligand to DNA bases, which provided the additional evidence for the intercalation of copper complex into DNA and was used to explain the fluorescence spectra.更多还原