【摘要】 选用观赏凤梨不同品种的老叶鞘、嫩叶鞘、顶芽、侧芽、茎段、叶片等不同组织作外植体 ,接种在诱导培养基为改良 MS+ 2 .0 mg· L- 1的 BA+ 5 .0 mg· L- 1的 2 ,4- D+ 0 .2 mg· L- 1的 IBA,及分化培养基为改良 MS+ 4 .0 mg· L- 1的 BA+ 0 .2 mg·L- 1的 IBA,培养 60 d进行统计 ,老叶鞘的愈伤组织三个品种平均诱导率为 74.6% ,丛生芽的诱导率平均数达 1 7.1 % ,次之是侧芽、顶芽 ,嫩叶鞘、茎段、叶片 ,后者能诱导出愈伤组织 ,不能诱导出丛生芽更多还原

【Abstract】 Older sheath, young sheath , apical buds,lateral buds, stem cuttings, and leaves from three cultivars of Bromelliads, were cultured on MS medium supplemented with 4.0 mg·L -1 of BA, 0.2 mg·L -1 of IBA, for 60 days .It showed that the average induction rate of calli older was 74.6%,the average inducton rate of shoots 17.1% of older sheath followed by laterad buds, apical buds, young sheathes, and stem, and the worst were the leaves,which could form culli but no shoots .The diferences among the various cultivas were great.更多还原