【摘要】 将乙肝病毒表面抗原 (HBs Ag)基因与 Ca MV3 5 s启动子及 nos终止子构建植物表达载体 p1 3 0 1 HBs,直接法转入根癌农杆菌 EHA1 0 5 (Agrobacterium tumefaciens) ,以该菌株介导叶盘法转化番茄 ,得到抗潮霉素的再生植株 .抗性苗总 DNA经 PCR、Southern斑点杂交证实目的基因已整合到番茄基因组中 ,EILSA检测证明在番茄中正确表达了乙肝表面抗原蛋白 .更多还原

【Abstract】 Plant binary expression vector p1301HBs, which contained CaMV35s promoter, HBsAg gene and Tnos, was directly introduced into A. tumefaciens EHA105. With "leaf disk" method, tomato plants medicated by EHA105 were transformed and hygromycin resistant plantlets were obtained. The results of PCR and Southern dot blotting of tomato genomic DNA demonstrated that the target gene was integrated into the genome of tomato plants. The examination of ELISA suggested the HBsAg gene was expressed correctly in transgenic tomato plants.更多还原