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对硝基酚磷酸酶pNPPase的基因克隆和序列分析
Cloning and Sequence Analysis of pNPPase
【摘要】 对硝基酚磷酸酶(p nitrophenylphosphatasepNPPase)是一种以对硝基酚磷酸盐(p nitrophenylphosphate)为底物的酶,被广泛地应用于核酸标记.通过菌落原位显色法成功地从脂肪芽孢杆菌(Bacillusstearothermophilus)中克隆到了编码耐热pNPPase的基因.序列分析表明它是一个编码255个氨基酸的酶.
【Abstract】 pnitrophenylphosphatase(pNPPase)is an enzyme with pnitrophenylphosphateas its substrate and is widely applied in nucleotide labeling. The cloning of the gene encoding thermostable pNPPase from Bacillus stearothermophilusby color screening to in situ is reported. The enzyme is composed of 255 amino acid residues according its DNA sequence.
【基金】 国家自然科学基金资助项目(30070161);上海市启明星计划资助项目(01QA14018)
- 【文献出处】 复旦学报(自然科学版) ,Journal of Fudan University , 编辑部邮箱 ,2002年06期
- 【分类号】Q785
- 【被引频次】2
- 【下载频次】41