【摘要】 大豆kunitz型胰蛋白酶抑制剂(SBTi A2)存在于种子中,2种kunitz型胰蛋白酶抑制剂Tia和Tid得到纯化,并对它们的pH稳定性和温度稳定性进行了比较研究,而且通过人工饲料喂养鳞翅目昆虫棉铃虫来研究SBTi A2的抗虫性.结果显示,棉铃虫的幼虫喂食含有Tia的人工饲料后,表现出生长延缓、体重减轻、羽化率降低、死亡率增加.由此显示了SBTi A2作为生物农药及利用转基因方法使作物提高抗虫性的应用前景.更多还原

【Abstract】 Soybean kuntiztype trypsin inhibitors (SBTi) are ubiquitous in soybean seeds and two kinds of SBTi- Tia and Tid were purified from different varieties of soybean seeds. Compare the pH stability and thermal stability of Tia and Tid then study the insectresistance of Tia by feeding Heliothis armgera Hubner, a kind of lepidopteran pest harmful to cotton. Heliothis armgera larvae which grow on artificial feed of Tia showed slow growth, light weight, low maturation, and high death rate, which indicates the further prospect of using SBTi as a biological chemical or as an insectresistant gene to be transferred into plants.更多还原