【摘要】 目的　探讨对常规病理检查漏诊的肺癌纵隔淋巴结转移病灶的诊断方法。方法　应用逆转录聚合酶链反应法 (RT PCR) ,检测pN0 1 期非小细胞肺癌患者 (NSCLC)纵隔淋巴结中MUC1基因mRNA的表达。结果　 5枚肺良性疾病的局部淋巴结无MUC1基因mRNA表达 ,5枚经病理检查证实有淋巴结转移癌的NSCLC纵隔淋巴结中均检测到MUC1mRNA表达。实验组 19例患者的 78枚纵隔淋巴结中有 6枚检测到MUC1mRNA表达 ,从而诊断为纵隔淋巴结隐匿转移。结论　应用RT PCR法检测纵隔淋巴结中MUC1基因mRNA的表达可以提高临床对肺癌纵隔淋巴结转移诊断的准确性更多还原

【Abstract】 Objective To evaluate the diagnostic method of occult metastasis to mediastinal lymph nodes (MLNs) in patients with non small cell lung cancer (NSCLC). Methods The mRNA expression of mucin 1(MUC1) gene, an epithelial tissue specific gene, was detected in dissected mediastinal lymph nodes by RT PCR assay. Seventy eight MLNs which had no malignant evidence on routine histopathologic examination were assessed in 19 patients with stage pN0 1 disease. Five regional lymph nodes from 5 patients with benign pulmonary diseases and 5 MLNs proved malignant by histopathology from 5 patients with NSCLC were also studied as negative and positive control respectively. Results The mRNA of MUC1 was not detected in any specimen of negative control group, whereas the mRNA was detected in all MLNs of positive control group. The mRNA in 6 out of 78 MLNs from 19 patients with pN0 1 disease was also detected, and occult metastasis was diagnosed. Conclusion Detection of MUC1 mRNA expression might be helpful to diagnose occult metastasis in MLN in patients with lung cancer, and RT PCR is superior to routine histopathologic examination in staging NSCLC.更多还原