【摘要】 目的　探讨心房钠尿肽 (ANP)对内毒素休克大鼠动脉血压的作用及可能的机制 .方法　大鼠静脉给予脂多糖(L PS,2 mg· kg- 1 )后立即静脉给予 ANP(2 μg· kg- 1 ) ,记录动物平均动脉血压 (MAP) ,检测血浆一氧化氮 (NO)和血浆内皮素 (ET)浓度的变化 .结果　给予 L PS的大鼠 MAP持续下降 ,至 4 h时 MAP下降到 (8.1± 2 .6 ) k Pa;ANP治疗组大鼠 MAP在给药初期的短暂下降后逐步回升 ,至 4 h时 MAP仍维持在 (13.4± 2 .9) k Pa,与 L PS组相比有显著差异 (P<0 .0 5 ) .与对照组比较 ,L PS组动物 4 h时血浆 NO和 ET浓度均显著升高 (P<0 .0 1) ;与 L PS组比较 ,ANP治疗组的动物在 4 h时血浆 NO水平和 ET浓度均显著下降 (P<0 .0 5 ) ,但仍明显高于对照组 (P<0 .0 1) .结论　 ANP对 L PS引起的动脉血压的持续下降有作用 ,可能是通过拮抗 ET的产生、降低 NO的分泌而起作用 ,也可能是通过其他的途径更多还原

【Abstract】 AIM To investigate the effect of atrial natriuretic peptide (ANP) on the regulation of arterial blood pressureand their potential mechanism in endotoxic shock rat. METHODS Mean arterial blood pressure (MAP) was recorded and nitric oxide (NO) and endothelin (ET) concentration of plasma was measured respectively after lipopolysaccharide (LPS) and LPS incorporate with ANP was injected into the veins of rats. RESULTS Administration of LPS elicited a persistent decrease in MAP (8.1±2.6) kPa at 4 h. ANP could maintain MAP at higher levels [(13.4± 2.9 ) kPa, at 4 h, P <0.05] after an transient decline when LPS was injected. NO and ET concentrations in plasma was evidently higher in the ANP therapia group than in the control group respectively ( P <0.01). Compared with the LPS group, NO and ET concentrations in plasma in the ANP therapia group all significantly decreased ( P <0.05). CONCLUSION ANP plays a role in the regulation of arterial blood pressure in endotoxic shock induced by LPS. The effect of ANP may be via decrease secretion of ET and NO, or may be through other unknown mechanisms.更多还原