【摘要】 目的　研究正常及网状内皮系统被封闭的家兔在急性高浓度氯气中毒后血浆纤维结合蛋白 (p FN)含量变化及其在肺损伤中的作用 .方法　 32只家兔随机分成 2大组 (正常组和网状内皮系统封闭组 ) :正常组又分为 :正常对照组和氯气中毒组 ;封闭组又分为对照组和封闭后中毒组 .戊巴比妥钠 iv麻醉后 ,气管插管 ,开胸行肺动脉主干插管以测压 .对照组动物手术后不行氯气中毒 .封闭组动物印度墨水 iv 4h后再进行上述实验 .中毒组动物吸入 5 m L·L- 1的氯气持续 2 0min.定时检测 p FN含量 (免疫火箭电泳法 )、肺动脉压力、呼吸功能指标 (频率、潮气量和动脉血氧分压等 ) .实验结束后 ,处死动物并取出肺脏以检测肺水指标并行组织学检查 .结果　氯气中毒后引起了严重的肺损伤 ,有大量的泡沫状分泌物自气管内涌出 ,肺干、湿质量均显著增加 ,出现了肺水肿 .动物中毒后 p FN的含量显著下降 .网状内皮系统被封闭 4h后p FN含量下降 ,氯气中毒后 ,被封闭的动物 p FN下降更明显 ,且中毒症状重 ,8例动物中有 4例在中毒后 80 min内死亡 ,其他组动物在 12 0 min内无死亡 .封闭组中毒前后与对照组相比肺动脉压力无显著改变 ,而呼吸频率加速 ,潮气量下降 .结论　氯气中毒引起了严重的肺损伤、肺水肿 .中毒后血浆内FN含量显著下降更多还原

【Abstract】 AIM To study mechanisms and significances of plasma fibronectin (pFN) concentration changes in normal and reticuloendothelial system enclosed rabbits following acute exposure to high concentration of chlorine gas by inhalation. METHODS Thirty two rabbits were divided randomly into 2 groups: 1. normal group, 2. reticuloendothelial system enclosed group (16 rabbits in each group). Eight rabbits in each group were anesthetized by venous injection of pentobarbital sodium and tracheostomy and pulmonary artery catheterization were performed, then the rabbits were exposed to high concentration chlorine gas 5 mL·L -1 for 20 min, as measured group; 8 additional rabbits in each group, which were similarly treated but not exposed to chlorine gas, served as controls. The concentration of pFN were measured by immune rocket electrophoresis. The pressure of pulmonary artery (Pa), airway pressure and tidal volume were continuously measured and recorded simultaneously. All rabbits were sacrificed and parameters of lung were measured in the end of experiments. RESULTS In both control groups, concentration of pFN was decreased slightly with time. On the other hand, the concentration of pFN were decreased significantly after exposure to chlorine gas. The reticuloendothelial system enclosed rabbits showed more severe in symptoms, and 4 rabbits died of severe lung injury in 80 min after the exposure. The pulmonary artery pressure did not change significantly following the chlorine exposure, but respiratory rate was accelerated immediately, and tidal volume decreased. By the end of experiments, hematocrit of blood and parameters of lung water were increased significantly also. Under light microscopy, pulmonary edema, microvascular bleeding were seen clearly. CONCLUSION Exposure to high concentration of chlorine gas inducing pulmonary edema is likely to the primary cause of decreased concentration of plasma FN. Injection of India ink via vein to rabbits decreases the pFN level and causes more severe lung injury following exposure to chlorine gas.更多还原