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噬菌蛭弧菌Bd-9五种保藏方法的比较研究
Comparative study on five preserve methods of Bdellovibrio bacteriovorus
【摘要】 作者对噬菌蛭弧菌Bdellovibriobacteriovorus的不同保藏方法进行了试验研究,并对其保藏效果进行了比较,结果表明,5种保藏方法中以冷冻干燥法保藏效果最好,21个月的复活率为100%,其次为4℃营养肉汤液保藏法和-20℃甘油保藏法,17个月复活率均为67%。
【Abstract】 Bdellvibrio Bacteriovorus was preserved by five methods. It was found that freeze drying was the best in the five kinds of preserved ways with revival rate of 100% in twenty-one month period, followed by 4℃ nutrient broth and 4℃ of frost glycerin storage with revival rate of 67% in seventeen month period.
【关键词】 噬菌蛭弧菌;
保藏;
冷冻干燥;
甘油;
营养肉汤;
【Key words】 Bdellvibrio bacteriovorus; preserve; freeze drying; glycerin; nutrient broth;
【Key words】 Bdellvibrio bacteriovorus; preserve; freeze drying; glycerin; nutrient broth;
【基金】 北京市自然科学基金资助项目(6972007)
- 【文献出处】 大连水产学院学报 ,Journal of Dalian Fisheries University , 编辑部邮箱 ,2002年04期
- 【分类号】Q93-3
- 【被引频次】20
- 【下载频次】376