【摘要】 目的　探讨重庆地区HCV流行的主要基因型。方法　提取 69份血清标本的HCVRNA ,通过逆转录套式PCR(RTnested PCR)扩增 5′端非编码区 (5′NCR) ,以 4种限制性内切酶BstUⅠ、NciⅠ、HaeⅢ、RsaⅠ对扩增产物进行限制性片段长度多态性分析 (RFLP) ,依据不同的酶切电泳图谱确定HCV基因型。结果　 69份HCV感染者血清标本有 60份可以通过上述方法进行基因分型 ,其中主要基因型是 1b,2a分别占 36 2 4 %和 31 88%。结论　HCV 1b ,2a仍然是重庆地区主要的基因型 ,但其所占比例不如中国其它地区高 ,说明HCV的分布呈地域依赖性更多还原

【Abstract】 Objective To investigate the majority HCV genotype in Chongqing area.Methods Abstracted HCV RNA from 69 HCV infectious serum specimens and HCV 5′non conding region(5′NCR) was amplified by reverse transcription nested PCR.The PCR products were digested by 4 restriction enzymes(BstUⅠ?NciⅠ?HaeⅢ and RsaⅠ) for restriction fragment length polymorphism(RFLP) analysis. HCV genotypes were identified according to different electrophoretogram patterns.Results Sixty serum specimens of 69 were identified by this assay, the major HCV genotypes is 1b(25/69) and 2a(22/69) in Chongqing area. Conclusion HCV 1b,2a are the major genotypes in Chongqing area, but the ratio is not so high as the rest area of China.更多还原