【摘要】 目的 :研究超极化激活电流 (hyperpolarization activatedcurrent,Ih)在慢性神经源性痛中的作用。方法 :以单侧L5和L6脊神经结扎制备神经源性痛大鼠模型 ,运用全细胞电压钳技术记录和分析背根神经节 (dorsalrootganglion ,DRG)神经元Ih 的表达和激活特征。结果 :脊神经结扎后 ,相应节段小直径DRG神经元表达Ih 的细胞比率升高 ,但大、中直径神经元Ih 没有改变 ,另外大、中、小直径神经元Ih 的活性增强。结论 :神经损伤导致DRG神经元Ih 的表达和电流活性发生改变 ,提示此种电流参与了神经源性痛“外周敏化”的发生更多还原

【Abstract】 SUMMARY Objective:To investigate the roles of hyperpolarization activated current ( I h) of dorsal root ganglion neurons in neuropathic pain. Methods: The neuropathic rat model was prepared by tight ligation of L 5 and L 6 spinal nerves and whole cell voltage clamp technique was applied to examine the changes of I h. Results: After nerve ligation, the incidence of small diameter neurons expressing I h was significantly increased, but the quantities of I h in large and medium diameter neurons remained unchanged. In addition, nerve injury significantly increased I h current activity in all types of DRG neurons. Conclusion: The expression and electrophysiological activity of I h increased in injured DRG neurons, which might serve as one of the mechanisms underlying peripheral sensitivity in chronic neuropathic pain.更多还原