【摘要】 目的 :观察东亚钳蝎毒 (BMK)对 K 5 6 2 /ADM细胞多药耐药性 (MDR)的逆转作用。方法 :MTT法 ,Hoechst33342和 PI荧光染色 ,荧光分光光度法和流式细胞分光光度术。结果 :K5 6 2 /ADM细胞对阿霉素 (ADM)具有明显的抗性 ,与 K5 6 2细胞相比 ,抗性倍数约为 33倍 ,而两者对BMK的 IC50 接近 ,差异无显著性 (P>0 .0 5 ) ;低毒剂量 BMK(10 .0 μg/m L,5 0 .0 μg/m L)与 ADM(4.0μg/m L)联合应用 ,可显著增强 ADM对该细胞的生长抑制和诱导凋亡作用 ,升高细胞内 ADM的浓度 ,降低该细胞对 ADM的 IC50 ,使该细胞对 ADM的抗性有数倍逆转。结论 :BMK可能是一种有效的MDR逆转剂 ,且与诱导细胞凋亡密切相关更多还原

【Abstract】 Objective:To observe the effect of BMK on reversal of multidrug resistance in K562/ADM cell line.Methods:MTT,Hoechst33342 and PI fluorescence test,fluorescence spectrophotometer and flow cytometry.Results:(1)K562/ADM cell line was more 33 fold resistant to ADM than their sensitive conterparts (K562 cell line),but the IC 50 value of both cell lines to BMK was nearly no difference ( P >0.05);(2)low cytotoxic dose (10.0 μg/mL,50.0 μg/mL) of BMK in combination with ADM (4.0 μg/mL) could enhance the effect of ADM on inhibition of cell growth and induction of apoptosis in K562/ADM cell line,increase the intracellular ADM concentration,decline IC 50 value of K562/ADM cell line to ADM.Conclusion:BMK might be an effective drug for reversal of MDR.Apoptosis and MDR are related.更多还原