【摘要】 以RP-HPLC对细胞培养液中紫杉醇的含量进行定量分析研究 ,并比较了 2种不同的定量方法———标准曲线法和绝对分析法 .实验结果表明 :绝对分析法与标准曲线法测定的紫杉醇的摩尔吸光系数及样品中紫杉醇质量浓度结果的偏差在 1.0 %左右 ,验证了绝对分析法的可靠性 .HPLC具有良好的线性关系 (r=0 .9999) ,加标回收率为 97.9%～ 10 2 .0 % ,相对标准偏差为 2 .1% ,最低检出限为 2 6 .0 μg·L-1更多还原

【Abstract】 A RP HPLC is developed for the quantitative analysis of the taxol in culture solution of plant cell. By comparison of two kinds of quantitative methods (absolute analysis method and standard calibration curves), the results show that the relative error of the determined ε and the sample concentration between the absolute analysis method and standard calibration curves is 1.0% or so.The reliability of absolute analysis method is validated. HPLC has a good linear relationship ( r =0.999 9), the recovery are 97.9%～102 0%,RSD is 2.1%,the detection limit is 26 μg·L -1 .更多还原