【摘要】 将聚磺酸酯EastmanAQ38(AQ)与细胞色素c (Cytc)的混合水分散系涂布于热解石墨电极表面 ,晾干后可形成Cytc AQ薄膜 .在pH 7.6的缓冲溶液中 ,Cytc AQ薄膜电极表现出良好的直接电化学行为 ,电化学活性中心为血红素Fe(Ⅲ ) /血红素Fe(Ⅱ )氧化还原对 .Cytc AQ薄膜在 - 0 .0 5V(vsSCE)处的一对可逆的循环伏安还原氧化峰随浸泡时间的延长而逐渐降低 ,而在 - 0 .4 0V的另一对峰逐渐增高 ,表明Cytc在AQ薄膜中很可能经历了由其原始构象向另一种新构象的转变 ,后者在AQ薄膜中十分稳定 .对其伏安行为进行了较详尽的研究 ,并估计了体系的电化学参数 ,如表观异相电子传递速率常数ks和式量电位E°′等 .Cytc AQ薄膜电极可催化还原溶液中的氧更多还原

【Abstract】 A mixture of an aqueous dispersion of poly (ester sulfonic acid) or Eastman AQ38 (AQ) and a solution of cytochrome c (Cyt c) is cast on the surface of pyrolytic graphite (PG) electrodes, and Cyt c AQ films are formed after being dried. In pH 7.6 buffers,Cyt c AQ film electrodes demonstrate good direct electrochemistry.The electroactive center of Cyt c in AQ films is the heme Fe(Ⅲ)/Fe(Ⅱ) redox couple. For Cyt c AQ films, the first pair of cyclic voltammetric peaks at -0.05 V(vs SCE) decreases gradually with soaking time while the second peak pair at -0.40 V increases at the same time, indicating possible transformation of Cyt c from its native conformation to a new one. The new conformation in Cyt c AQ films is quite stable in solutions, and its voltammetric behavior is studied in detail. The electrochemical parameters of the system, such as the apparent heterogeneous electron transfer rate constant ( k s) and formal potential ( E °′), are estimated. Cyt c AQ films can catalyze the reduction of oxygen in solutions.更多还原