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用酪氨酸酶生物传感器测水中酚类物质的研究
RESEARCH OF TYROSINASE BIOSENSOR FOR DETECTION OF PHENOLS
【摘要】 研究了用麦芽糊精修饰的酪氨酸酶碳糊电极构成电流型生物传感器测水中酚类污染物质的方法 .在外加电压为 - 1 0 0 m V( vs.SCE) ,p H值为 5 .40的磷酸盐缓冲溶液中 ,在浓度为 2 .0×1 0 -7~ 1 .0× 1 0 -5mol/L的范围内电极与苯酚的浓度有良好的线性关系 ,检出下限为 1 .0× 1 0 -7mol/L ,响应时间为 2 min.此电极对其他酚类物质如 :邻苯二酚 ,对氯苯酚 ,邻甲酚等都有良好的响应 .利用此种电极可用来检测工业废水中的酚类物质的含量
【Abstract】 Discussed a method for measuring phenols with a maltodextrin modified amprometric biosensor based on tyrosinase carbon paste electrode. When the working potential was -100 mV (vs.SCE),pH was 5 40 in the phosphate buffer solution and the time for measurement was 2 min,the detection limit was 1 0×10 -7 mol/L and the linear range was 2 0×10 -7 ~5 0×10 -5 mol/L. This method was also suitable for detection of catechol, o cresol and para chlorophenol, etc. This electrode could be applied to monitor the concentration of phenols in industry wastewater.
【Key words】 biosensor; amprometric enzyme electrode; tyrosinase; maltodextrin;
- 【文献出处】 北京工商大学学报(自然科学版) ,Journal of Beijing Institute of Light Indusry , 编辑部邮箱 ,2002年01期
- 【分类号】X132
- 【被引频次】25
- 【下载频次】372