【摘要】 利用间接ELISA对感染WYMV的小麦病叶检测 ,通过比较分析影响ELISA测定结果的包被缓冲液、抗血清及酶标抗体的浓度和种类 ,确立了较为稳定的反应体系。利用此ELISA检测体系并结合RT PCR方法对从四川采集的田间小麦中WYMV进行检测 ,确定了有较高的检测灵敏度的检测系统 ,为田间大量小麦病样的检测提供有效、方便的方法更多还原

【Abstract】 WYMV was detected by ID ELISA(Indirect ELISA) Different experiment conditions of ELISA on WYMV were compared, for example, types and concentrations of buffer, different antiserums, different IgG and so on The most effective ID ELISA system was developed RT PCR revealed that this ELISA system is efficient to detect WYMV in wheat更多还原