【摘要】 目的　对各类输血反应进行调查研究 ,同时建立输血前免疫血液学检查新技术 ,以预防和治疗免疫性输血反应。方法　对上海地区 13家医院的 30 776名输血病人进行调查 ,并应用改良Polybrene、简易致敏红细胞血小板血清学试验 (SEPSA)等新技术 ,进行同种特异性抗体的筛选、鉴定及交叉配型试验。结果　发生各类输血反应5 0 8例 (1.6 5 % ) ,其中检出引起免疫性输血反应的同种抗体 6 4例 (12 .6 % ) ,抗体特异性分别为血小板抗体 (包括HLA) 5 1例 ,红细胞不规则抗体 13例 ;各项新技术的应用对预防免疫性输血反应的发生具有显著相关性 (χ2 >3.84 ,P <0 .0 5 )。结论　使用研究建立的输血前免疫血液学新技术能有效地诊断、预防和治疗免疫性输血反应的发生 ,提高了输血的安全性和有效性更多还原

【Abstract】 Objective Analysis of transfusion reaction data based on patients in Shanghai region.Establishment of modified pre transfusion testing strategies to reduce immune transfusion reactions.Methods We retrospectively analyzed records of transfusion patients ( n =30776) from 13 hospitals in Shanghai.In this study we used manual Polybrene test and Simplified Sensitized Erythrocyte Platelet Serology Assay (SEPSA) test to determine the red cell and platelet alloantibodies.Results The total cases of transfusion reaction were 508(1.6%).Among these cases,alloantibodies have been identified in 64 patients(12.6%),including 51 platelet and HLA antibody and 13 red cell alloantibody.As compared with the present methods of pre transfusion test, manual Polybrene test and SEPSA test will efficiently reduce immune transfusion reactions(χ 2>3.84, P <0.05).Conclusion These observations suggest that new immunohematological methods appear effective for preventing,diagnosing and treating transfusion reactions.更多还原