【摘要】 探讨灵芝中酸性组分的提取、分离及分析方法 ,并研究其抑菌活性 .结果表明 ,灵芝醇提物经碱处理分离出总酸性组分的产率为 0 .8%～ 1.1% ,当以 n- C6 H1 4/ CH2 Cl2 / CHCl3/ Me OH(体积比为 4 .0∶ 3.0∶ 2 .5∶ 0 .5 )为展开剂时 ,薄层色谱仅显示 Rf 值为 0 .5 4～ 0 .12的 6条主要带 (S1 ～ S6 ) ;抑菌圈法测定结果显示 :该灵芝的酸性组分为2 5 m g/ m L时对金黄色葡萄球菌 (G+ )和大肠杆菌 (G- )均有明显抑制作用 ;粗灵芝酸性组分经硅胶柱层析纯化后 ,CHCl3/ Me OH (V∶ V=98∶ 2 )洗脱部分 (S1 )对大肠杆菌有明显抑制作用 ,而对金黄色葡萄球菌呈明显抑制作用的则集中在 CHCl3/ Me OH(V∶ V=5 0∶ 5 0 )洗脱部分 (S5,S6 ) .上述灵芝酸性组分的提取与分离方法简便易行 ,成分与产率稳定可控 ,且有明显抗菌活性 ,在抗菌制剂应用方面有潜在的药用价值 .更多还原

【Abstract】 Methods of extraction, separation, identification and about the antibacterial activity of acidic components from Ganoderma lucidum are investigated. The results showed that the yield of acidic components was 0 8%～1 1% of dry Ganoderma lucidum , when isolated by alkalinization from alcohol extract. Using n C 6H 14 /CH 2Cl 2/CHCl 3/MeOH(the volume ratio is 4 0∶3 0∶2 5∶0 5) as the mobile phase on silica gel film, the acidic components were identified as six major spots (S 1～S 6), with their R f values ranging from 0 54 to 0 12. The antimicrobial circle test showed that the acidic components at a concentration of 25 mg/mL had obvious action in bacteriostasis upon S.Aureus (G +) and E.coli (G -). Elution with CHCl 3/MeOH( V∶V =98∶2)(S 1) had bacteriostasis on E.coli , while elution with CHCl 3/MeOH( V∶V =50∶50)(S 5,S 6) exhibited bacteriostasis on S.Aureus . The above methods for extraction, separation and identification of acidic components from G.lucidum are simple and efficient. Furthermore, the obtained acidic components are controllable and bacteriostatic, and these may have potential medicinal application in antibacterial reagents.更多还原