Methods of extraction, separation, identification and about the antibacterial activity of acidic components from Ganoderma lucidum are investigated. The results showed that the yield of acidic components was 0 8%~1 1% of dry Ganoderma lucidum , when isolated by alkalinization from alcohol extract. Using n C 6H 14 /CH 2Cl 2/CHCl 3/MeOH(the volume ratio is 4 0∶3 0∶2 5∶0 5) as the mobile phase on silica gel film, the acidic components were identified as six major spots (S 1~S 6), w...