节点文献
家蚕质多角体病毒(BmCPV)基因组dsRNA片段V的全序列测定
Sequencing of Genomic dsRNA Segment V of Bombyx mori Cytoplasmic Polyhedrosis Virus
【Abstract】 dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of them
【Key words】 Bombyx mori cytoplasmic polyhedrosis virus(BmCPV); dsRNA; RT-PCR; sequencing;
- 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,2002年04期
- 【分类号】S884.5
- 【被引频次】9
- 【下载频次】94