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家蚕质多角体病毒(BmCPV)基因组dsRNA片段V的全序列测定

Sequencing of Genomic dsRNA Segment V of Bombyx mori Cytoplasmic Polyhedrosis Virus

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【作者】 林伟徐安龙杨文利孙京臣卢火斤英张景强

【Author】 LIN Wei1,XU An-long2,YANG Wen-li2,SUN Jing-chen1,LU Xin-ying1,ZHANG Jin-qiang1trol,Zhongshan University,Guangzhou 510275,China;2The National Opening Laboratory for Marine Functional Genomics Research,Zhongshan University,Guangzhou 510275,China)

【机构】 中山大学生物防治国家重点实验室中山大学海洋生物功能基因组开放实验室中山大学生物防治国家重点实验室 广州 510275广州 510275广州 510275广州 510275

【Abstract】 dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of them

【基金】 国家自然科学基金结构生物学倾斜项目(No 30070169)
  • 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,2002年04期
  • 【分类号】S884.5
  • 【被引频次】9
  • 【下载频次】94
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