【摘要】 为筛选用于我国HIV疫苗的候选基因 ,利用痘苗病毒载体构建表达HIV - 1B′亚型中国流行株RL4 2 的Gag蛋白的重组病毒rVV -RL4 2 gag ,并免疫BALB/c小鼠 ,检测其诱导的特异性抗体及中和抗体 ,同时检测其诱导的特异性CTL及与中国流行株C(B′/C重组 )亚型和国际流行株A亚型之间的CTL交叉反应。结果显示 :重组病毒能很好地表达Gag蛋白 ,它具有与 7株单克隆抗体结合的抗体表位 ;电镜下可观察到Gag蛋白形成的颗粒。rVV -RL4 2 gag免疫小鼠后能诱导产生高滴度的特异性抗体和CTL ,抗体具一定的中和活性 ,且检测到与C(B′/C重组 )亚型和国际流行株A亚型之间的CTL交叉反应。这些结果表明 ,rVV -RL4 2 gag具有良好的免疫原性 ,可进一步构建表达HIVB′亚型中国流行株RL4 2 的Gag蛋白的重组痘苗病毒作为候选疫苗。更多还原

【Abstract】 A recombinant vaccinia virus containing the gag gene of Chinese epidemic HIV-1 strain(RL 42 ,clade B′), under the control of vaccinia virus promoter p7.5,was constructed by inserting the gag gene into the TK region of vaccinia virus. Through ELISA and Western blot assays,it was shown that the recombinant rVV-RL 42 gag could successfully express the HIV p55.Gag protein had the epitopes capable of binding seven monoclonal antibodies,and could assemble into particles that could be detected by electron microscope.The recombinant vaccinia virus rVV-RL 42 gag could induce humoral and CTL immune responses after inoculating BALB/c mice, the specific and neutralization antibodies could be detected.The CTL induced by recombinant virus could cross-react with clade A,B′and C(B′/C)peptide epitopes.The results suggested that recombinant vaccinia virus containing the gag gene of Chinese HIV-1 strain(RL 42 ,clade B)can be constructed as a candidate HIV-1 vaccine of China.更多还原