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PET15b-BCG HSP65-GP2重组质粒的构建与鉴定
Construction of recombinant plasmid expressed PET15b-BCG HSP65-GP2 fusion protein gene
【摘要】 目的 :构建表达卡介苗热休克蛋白 6 5 (BCGHSP6 5 )与人HER 2 /neu细胞毒T淋巴细胞 (CTL)表位 (GP2 )构成的融合蛋白 (BCGHSP6 5 GP2 )的重组质粒。方法 :将用PCR方法扩增并回收的BCGHSP6 5 GP2 基因片段与原核表达质粒PET15b重组 ,经过酶谱分析筛选出正向重组质粒 ,DNA序列分析正向重组质粒。结果 :筛选出 3个正向重组质粒。结论 :重组表达质粒PET15b BCGHSP6 5 GP2 的构建是成功的 ,为进一步表达奠定基础
【Abstract】 Objective:To construct a recombinant plasmid that expressed BCG HSP65 fused with human HER 2/neu cytotoxic T lymphocyte(CTL) epitope.Methods:BCG HSP65 GP 2 gene fragment was amplified by PCR approach and then cloned into prokaryote expressed plasmid PET 15b .Forward recombinant plasmids were screened by endo nucleases digestion analysis.Results:Three forward recombinant plasmids were obtained.Conclusions:It has been suggested that we have constructed a recombinant expressed PET 15b vector which expressed BCG HSP65 fused with human HER 2/neu CTL epitope.It lay foundation on further expression and biofunction assay.
【Key words】 gene expression regulation； oncogen products； heat shock proteins； plasmid； HER 2/neu；
- 【文献出处】 蚌埠医学院学报 ,Journal of Bengbu Medicinae College , 编辑部邮箱 ,2002年05期
- 【分类号】R73-362
- 【被引频次】1
- 【下载频次】49