【摘要】 目的 :用小鼠建立慢性皮炎 湿疹模型。方法 :用 7%二硝基氯苯 (DNCB)外涂小鼠背部致敏 ,5天后用 0 .1%DNCB于小鼠右耳内侧反复激发引起慢性皮炎的模型 ,每隔 3天激发 1次 ,对照组于激发后外用氢化可的松 ,两组进行对比观察。结果 :实验组小鼠耳部出现肿胀增厚、表面结痂、炎性细胞浸润增多 ,对照组的变化则不明显。结论 :小量DNCB反复刺激小鼠耳部可建立慢性皮炎 湿疹模型 ,而外用氢化可的松后可抑制该模型成立更多还原

【Abstract】 Objective:To establish the model of chronic dermatitis eczema in mice.Methods:Right ears of CXA 1 mice which were previously sensitized with 7% dinitrochlorobenzen(DNCB) were repeatedly challenged with 0.1% DNCB for five time.The controls were applied hydrocortisone after each challenge.Results:After 4th and 5th challenges,changes such as ear swelling thickening,crusts and cellular infiltrates in dermis were observed,and these features seemed to be more significant in vehicle treated mice compared to hydrocortisone controls.Conclusions:Repeated challenges with a small dose of DNCB may induce chronic dermatitis eczema in mice,while application of hydrocortisone can inhibit these responses.更多还原