【摘要】 背景与目的:许多研究报告表明,茶多酚(TP)有防癌和抗癌作用,但对鼻咽癌细胞作用的报道很少。我们最近研究证明TP对人鼻咽癌CNE2细胞有抗增殖和诱导凋亡的作用。为进一步了解TP对人鼻咽癌细胞的作用,进行TP对多种鼻咽癌细胞和人鼻咽癌裸鼠移植瘤生长影响的实验研究。方法:以MTT法检测TP对NPC/HK1、CNE1、CNE2、HNE1、HNE2、SUNE1和Fadu7种人鼻咽癌细胞株的生长抑制作用。以人鼻咽癌细胞株(CNE2)裸鼠移植瘤为模型,作体内抑瘤试验。结果:在体外抗癌细胞增殖试验中,TP对NPC/HK1、CNE1、CNE2、HNE1、HNE2、SUNE1和Fadu的IC50分别为55.83、98.43、119.21、127.74、158.07、160.72μg/ml和163.59μg/ml。在TP对人鼻咽癌细胞株(CNE2)裸鼠移植瘤的抑瘤试验的3批实验中,5mg·(kg·d)-1TP作用18天的平均抑瘤率为12.7%,P>0.05;10mg·(kg·d)-1TP作用18天的平均抑瘤率为31.0%,P<0.01;20mg·(kg·d)-1TP作用18天的平均抑瘤率为38.5%,P<0.01。结论:TP对7种人鼻咽癌细胞株均有不同程度的生长抑制作用,对人鼻咽癌CNE2裸鼠移植瘤的生长有一定的抑制作用。更多还原

【Abstract】 Background and Objective:It was reported that tea polyphenol(TP) possess preventive and anticancer effects on various human cancers. However the report about TP against human nasopharyngeal carcinoma(NPC) was very rare. Our previous studies have also suggested that TP has antiproliferative effect and may induce apoptosis in human NPC cell. In order to further explore the antitumor effect, the authors investigated the growth inhibition effect of TP on various human NPC cell lines and xenograft tumors of human NPC in nude mice.Method: Antiproliferative effect of TP against seven human NPC cell lines was tested by MTT method. Antitumor effect of TP was determined using the xenografts models of human NPC cell (CNE2) in nude mice. Results: The fifty percent inhibition concentration (IC50) of TP against NPC cell lines (NPC/HK1, CNE1, CNE2, HNE1, HNE2, SUNE1,and Fadu cells) were calculated to be 55.83, 98.43, 119.21, 127.74, 158.07, 160.72,and 163.59μg/ml, respectively. Average inhibitory rates of TP against xenograft tumors of human NPC in nude mice were 12.7%(P > 0.05), under the dosage of 5 mg·(kg.·d) 1, ig×18 d. Under the dosages of 10 mg·(kg.·d) 1, ig×18d and 20 mg·(kg.·d) 1, ig×18 average inhibitory rates were 31.0%and 38.5%,(P< 0.01),respectively. Conclusion: The results indicated that TP possessed different antiproliferative effect on seven human NPC cell lines in vitro and on xenograft tumor of human NPC in nude mice.更多还原