【摘要】 背景与目的:缺氧诱导因子-1(Hypoxiainduciblefactor-1,HIF-1)是调节肿瘤细胞适应缺氧的核转录因子。大量研究证实HIF-1在维持肿瘤细胞的能量代谢、新血管形成及转移起重要作用。但关于HIF-1与肿瘤细胞凋亡、增殖的关系鲜见报道,且结论存在较大差异。本研究旨在探讨缺氧诱导因子-1α(HIF-1α)在肺癌组织中的表达及其与bcl-2、Bax及PCNA的关系。方法:采用免疫组化SP法检测60例肺癌组织中HIF-1、bcl-2、Bax蛋白及PCNA的表达。结果:60例肺癌组织中HIF-1α阳性表达率为28.3%。其中小细胞肺癌(SCLC)阳性率为66.7%显著高于非小细胞肺癌(NSCLC)的21.5%(P<0.01)。HIF-1α表达水平随临床分期的增高而增加(P<0.01)。bcl-2Bax,PCNA蛋白阳性率分别为31.7%(19/60),40.0%(24/60),76.7%(46/60)。bcl-2的表达与肿瘤细胞的分化程度显著相关(P<0.01)。HIF-1α蛋白表达与bcl-2呈负相关(P<0.01),与Bax蛋白之间显著正相关(P<0.01),但与PCNA无相关性(P>0.05)。结论:HIF-1α与肿瘤细胞凋亡密切相关,但与肿瘤细胞增殖无关。更多还原

【Abstract】 Background &Objectives:Hipoxia-inducible factor-1is a tra nscriptive factor that regulates ge nes involved in metabolism,angiogenesis,proliferation,and apoptosis.This study was designed to investigate the expr ession of hypoxia inducible facter-1alpha(HIF-1α)and its relationship to bcl-2,Bax,PCNA in lung cancer.Method:Immunohistochemical streptavidin /peroxidase(SP)was used to examine the expression of HIF-1a,bcl-2,Bax,and PCNA in 60cases of lung cancer.Results:In 60cases of lung cancer,postive ra te for HIF-1a was 28.3%(17/60),specially the postive rate of small cell lung cancer(66.7%)was significantly higher than non-s mall cell lung cancer(21.6%).HIF-1a expression increased as clinical stage and meta stasis increased(P <0.01).The positive rate of bcl-2,Bax,and PCNA were 31.7%(19/60),40.0%(24/60),76.7%(46/60),respectively.Inverse relationsh ip was found between the expression o f HIF-1αand bcl-2;while the correlation of H IF-1αand Bax was positive(P<0.01).The relationship between HIF-1αand Bax was postive(P<0.01).The relationship between HIF-1αand PCNA was not observed(P>0.05).Conclusion:HIF-1αis correlated with apopotosis,but has no relation ship with proliferation.更多还原