【摘要】 目的 :揭示电针治疗在缺血性脑血管疾病中的分子机制。方法 :采用原位标记法 ,研究电针督脉的大椎、百会经穴对缺血性脑损伤的保护作用。凝闭大鼠大脑中动脉致局灶性脑缺血 10h后 ,选择末端脱氧核苷酸转移酶介导dUTP缺口末端标记法 (TUNEL)检测神经细胞的凋亡状况 ,并观察电针对其影响。结果 :电针组大脑皮质梗死区内TUNEL染色阳性细胞显著减少 ,与模型组比较 ,差异有显著性意义 (P <0 .0 1)。结论 :电针可抑制脑缺血后脑内神经细胞凋亡。更多还原

【Abstract】 Objective:To research the molecular mechanism of electro-acupuncture (EA) in the treatment of ischemic cerebral vascular diseases. Method: By using in situ labeling technology,the effects of electro-acupuncturing Dazhui (Du 14),Baihui (Du 20) on ischemic cerebral injury were observed. 10 hours after focal cerebral ischemia induced by coagulation of rat middle cerebral artery, by using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL), the effects of EA on neurocyte apoptosis were observed. Result: The TUNEL staining positive cells of ischemic region of cortical layer in EA group were significantly decreased as compared with those in model group (P<0.01). Conclusion: Acupuncture can restrain the neurocyte apoptosis induced by ischemic cerebral injury.更多还原