【摘要】 目的 :研究以荧光原位杂交 (fluorescenceinsituhybridization ,FISH)技术评价了中草药昆明山海棠根部水抽提物[Tripterygiumhypoglaucum (Level)Hutch ,THH]在小鼠骨髓细胞中的特异染色体不分离效应。方法 :以受试物THH腹腔注射昆明种雄性小白鼠 ,2 4h后取骨髓细胞常规制片 ,以bio 16 dUTP标记的 8号染色体特异性着丝粒重复顺序探针进行FISH ,并以streptavidine Cy3与杂交信号结合。荧光显微镜下分析受试物处理动物后骨髓细胞 8号染色体分离情况。结果 :在3个受试剂量中 ,THH具有与阳性对照秋水仙素类似的作用趋势 ,其所诱发的 8号染色体不分离频率均显著高于溶剂对照。结论 :本研究证实了THH为小鼠骨髓细胞 8号染色体异常分离的诱发因素。更多还原

【Abstract】 Purpose: The water extract of a Chinese herb, Tripterygium hypoglaucum (Level) Hutch (THH) was assayed for its effects of aneuploidy induction in mouse bone marrow cells by means of fluorescence in situ hybridization (FISH). Methods: Kunming species mice were injected with THH into the abdominal cavity and killed at 24 h after treatment. The bone marrow cell slides were prepared by general methods. FISH was carried out with Bio 16 dUTP labbled chromosome 8 probe. The hybridization signals were detected by combining streptavidine Cy3. Results: In three dose groups THH induced chromosome 8 aneuploidy and the aneuploidy frequencies of chromosome 8 were significantly higher than the solvent control (P<0.001～0.05). Conclusion: The result indicated that THH is an aneugen of chromosome 8 in mouse somatic cells.更多还原