【摘要】 目的　观察抑肽酶对家兔全脑缺血再灌注损伤的保护效果。方法　健康家兔 16只 ,体重 2 .0～ 2 .5kg ,随机分为抑肽酶处理组 (A组 )和缺血组 (C组 ) ,每组 8只。利用“六血管”式模型阻断动脉 30min ,恢复血流再灌注 4h。A组于缺血前 10min静注抑肽酶 30 0 0 0U·kg-1,继之以微量泵输注 10 0 0 0U·kg-1·h-1,在缺血前 15min(Ⅰ0 )和再灌注 4h(R4 )采颈内静脉血测定血清IL 8含量。C组不用药 ,阻断和再灌注时间及血样检测同A组。两组实验结束前活体取左颞皮层组织 ,一部分组织在光镜下观察白细胞浸润程度并计数 ,另一部分组织作Nissl’s小体平均光密度分析。结果　A组IL 8在R4 时增加 1.9倍 ,同缺血前比有显著性差异 (P <0 .0 5 ) ;C组IL 8在R4 时增加 3.7倍 ,同缺血前比有显著性差异 (P <0 .0 1)。组间相比 ,A组IL 8升高幅度明显低于C组 (P <0 .0 5 )。光镜下 ,A组皮层组织白细胞浸润的数量平均为 2 1± 6个 (9～36个 ) ;C组白细胞浸润数量明显增多 ,平均为 49± 13个 (16～ 98个 ) ,组间相比有显著性差异 (P <0 .0 5 ) ,并且有典型的血管套现象。A组Nissl’s小体平均吸光度为 0 .10 7± 0 .0 2 2 ,C组为 0 .0 44± 0 .0 0 8,组间相比有高度显著性差异 (P <0 .0 1)。结论　抑肽酶能抑制脑缺血再灌注期IL更多还原

【Abstract】 Objective To determine the protective effect of aprotinin on the brain injuries following cerebral ischemia reperfusion in a rabbit.Methods Sixteen rabbits were divided randomly into group C with cerebral ischemia induced by clipping of bilateral external, internal carotid arteries and vertebral arteries for 30 min and then with the reperfusion lasting 4 hours;group A with aprotinin (30000 U·kg -1 ) 10 min before occlusion and followed 10000 U·kg -1 ·h -1 intravenously till the end of procedure.A catheter was inserted into internal jugular vein and positioned so that the tip was in the jugular bulb.Blood samples were obtained through the catheter, the tip of which was positioned at the jugular bulb.The serum concentration of IL 8 was measured 15 min before occlusion(Ⅰ 0), 4 hours (R 4) after reperfusion.Under light microscopy, the degree of leukocyte infiltration and average optical density of the Nissl’s corpuscles in cerebral tissue were analyzed.Results The level of IL 8 decreased remarkably in group A compared with that in group C at R 4 ( P < 0.05 ).IL 8 levels in group A and C were 1.9 and 3.7 times higher than that at R 4 respectively.Under light microscope, the number of leukocyte infiltration in group A and C were 21±6(9-36), 49±13(16-98).The degree of leukocyte infiltration decreased obviously in group A than in group C.Besides, there was typical vessel sheath in the group C.The average optical density of the Nissl’s corpuscles in group A and C were 0.107 ± 0.022 and 0.044 ± 0.008 respectively, there were significantly difference between group A and C.Conclusion Aprotinin attenuated the brain reperfusion injury, through suppresses effectively the production of IL 8 and leukocyte activation.更多还原