【摘要】 目的 :对酶解小鼠卵巢 ,获取初级卵泡的方法进行探讨。方法 :卵巢取自性成熟前的昆明系小白鼠 ,酶液由不同浓度的胶原蛋白酶、脱氧核糖核酸酶和链蛋白酶组成 ,培养液为WaymouthmediumMB75 2 /I,并添加牛血清白蛋白、胰岛素和转铁蛋白。实验共分 6组 ,每组所用酶液组成和处理时间各不相同。结果 :由 3mg/ml的胶原蛋白酶、10 0IU/ml的脱氧核糖核酸酶 ,另加或不加 0 .0 2mg/ml的链蛋白酶组成 ,处理时间为 2 0分钟的两个处理组 ,卵泡分离效果较好。结论 :胶原蛋白酶浓度是影响卵巢中卵泡分离的主要因素 ,它和消化时间对卵泡分离及分离后卵泡形态、存活能力有影响。更多还原

【Abstract】 The present study was carried out to evaluate six enzymatic methods(based on enzymatic concentration)to dissociate of primary follicles from mouse ovaries and observe their in vitro development.The results showed that the medium containing 3mg/ml collagenase and 100IU/ml DNAase with or without 0.025mg/ml pronase E obtained the best efficiency among the six groups.It indicated that the concentration of collagenase in the medium would be a key factor for isolating of primary follicles.更多还原