【摘要】 目的 :研究冰片对大鼠脑微血管内皮细胞ICAM -1表达量的影响。方法 :采用自由落体撞击硬脑膜致伤方法制成大鼠脑挫裂伤模型 ,将大鼠伤侧脑皮质制成石蜡切片 ,运用免疫组化学染色技术观察空白对照组 (C组 :只灌服石蜡油 )、冰片组 (B组 :灌服等量冰片石蜡油 )、脑外伤组 (M组 :脑挫裂伤组 )和脑外伤加冰片组 (M +B组 :脑挫裂伤大鼠灌服冰片石蜡油 )大鼠脑皮质微血管内皮细胞 (EC)膜表面细胞间粘附分子 -1 (ICAM -1 )量的表达 ,并进行半定量分析。结果 :C组EC无表达或有少量表达 ,服用冰片不能使I CAM -1表达增强 ,脑外伤后ICAM -1表达有明显增强 (P <0 .0 0 1 ) ,及脑外伤大鼠服用冰片可使ICAM -1表达明显减弱。结论 :证明冰片促血脑屏障 (BBB)开放与ICAM -1无关 ,冰片对脑外伤大鼠脑组织具有一定的保护作用更多还原

【Abstract】 Methods: The experimental brain injury was induced in the SD rats by free falling object, and the expression of intercelluar adhesion molecule-1(ICAM-1)in the microvascular endothelial cells (EC) of rat brain was examined by immunohistochemistry and S-P histochemical methods.The rats were allocated to group A(normal blank control group), group B (administration of Borneolum), group C(brain injured model group) and group D(model rats treated with Borneolum).Results:ICAM-1 seldom expressed or never expressed in the normal rats brain,the administration of Borneolum could not induce its expression,and ICAM-1 expression increased in the injured brain(P<0.001).Conclusion:Borneolum inducing blood-brain barrier opening has no relationship with the expression of ICAM-1, and Borneolum exerts certain protective effect on the injured rat brain.更多还原