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EFFECT OF LOW DOSE IRRADIATION ON EXPRESSION OF MEMBRANE MOLECULES OF T LYMPHOCYTES IN CORD BLOOD

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【Author】 Cancer Treatment Center, Peking University Third Hospital, Beijing 100083, China(LIU CA, YANG G, JIA TZ); Peking Univeristy Health Science Center, Beijing 100083, China (GUAN ZW, ZHANG L)

【机构】 Cancer Treatment Center； Peking University Third Hospital； Beijing 100083； China； Peking Univeristy Health Science Center； China；

【摘要】 <正> To study on the effect of low dose irradiation （LDI） on expression of membrane molecules of T lymphocytes in cord blood.Methods Freshly isolated lymphocytes from cord blood were irradiated with 62mGy gamma-ray. At different times （4h, 12h, 24h） after irradiation the changes of TCR⁺, CD₃⁺, CD₄⁺ and CD₈⁺ cells were examined by flow cytometry with direct immunofluorescence respectively.Results The proportion of CD₃⁺, TCR⁺/CD₃⁺, CD₄⁺ and CD₈⁺ cells increased significantly after LDI, with the most obvious enhancement noted in the 24h experimental group. The ratio of CD₄ to CD₈ showed no significant changes.Conclusion It is suggested that expedition of the maturation, activation and signal transduction of T lymphocytes from cord blood can be induced by irradiation of 62mGy gamma-ray. So the reconstruction of immune functions after cord blood transplantation can be accelerated, thus enhancing the effect of graft versus leukemia（GVL） and preventing the tumor from relapsing.更多还原

【Abstract】 To study on the effect of low dose irradiation （LDI） on expression of membrane molecules of T lymphocytes in cord blood.Methods Freshly isolated lymphocytes from cord blood were irradiated with 62mGy gamma-ray. At different times （4h, 12h, 24h） after irradiation the changes of TCR⁺, CD₃⁺, CD₄⁺ and CD₈⁺ cells were examined by flow cytometry with direct immunofluorescence respectively.Results The proportion of CD₃⁺, TCR⁺/CD₃⁺, CD₄⁺ and CD₈⁺ cells increased significantly after LDI, with the most obvious enhancement noted in the 24h experimental group. The ratio of CD₄ to CD₈ showed no significant changes.Conclusion It is suggested that expedition of the maturation, activation and signal transduction of T lymphocytes from cord blood can be induced by irradiation of 62mGy gamma-ray. So the reconstruction of immune functions after cord blood transplantation can be accelerated, thus enhancing the effect of graft versus leukemia（GVL） and preventing the tumor from relapsing.更多还原

【关键词】 cytometry； irradiation； noted； graft； relapsing； gamma； versus； labeled； transduction； proportion；

【文献出处】 China Medical Abstracts(Internal Medicine) ,中国医学文摘(内科学分册)(英文版) , 编辑部邮箱 ,2001年03期

【分类号】R73-3
【下载频次】11

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